siRNA Pathway

Description:

Small interfering RNAs (siRNAs) are 21–23nt dsRNA (double-stranded RNA) molecules that facilitate potent and sequence-specific gene suppression via the mechanism of RNAi (RNA interference). When introduced into cultured mammalian cells, siRNAs facilitate the degradation of mRNA sequences to which they are homologous, thereby silencing the encoding gene. The basic mechanism behind RNAi is the breaking of a dsRNA matching a specific gene sequence into short pieces of siRNA. These siRNAs with symmetric 2–3nt 3'...

References:

Killing the messenger: short RNAs that silence gene expression.
Dykxhoorn DM, Novina CD, Sharp PA
Nat Rev Mol Cell Biol. 2003 Jun; 4(6): 457-67. Review.
Molecular mechanisms of Dicer: endonuclease and enzymatic activity.
Song MS, Rossi JJ.
Biochem J. 2017 May 4;474(10):1603-1618.
RNAi expression vectors in mammalian cells.
Miyagishi M, Taira K
Methods Mol Biol. 2004; 252:483-91.
Generation of transgenic Drosophila expressing shRNAs in the miR-1 backbone.
Chang K, Marran K, Valentine A, Hannon GJ.
Cold Spring Harb Protoc. 2014 May 1;2014(5):10.1101/pdb.prot080762 pdb.prot080762.

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Pathways

siRNA Pathway

Description:

References:

Killing the messenger: short RNAs that silence gene expression.

Molecular mechanisms of Dicer: endonuclease and enzymatic activity.

RNAi expression vectors in mammalian cells.

Generation of transgenic Drosophila expressing shRNAs in the miR-1 backbone.

Databases

Tools

Institutional License

Pathways

siRNA Pathway

Description:

References:

Killing the messenger: short RNAs that silence gene expression.

Molecular mechanisms of Dicer: endonuclease and enzymatic activity.

RNAi expression vectors in mammalian cells.

Generation of transgenic Drosophila expressing shRNAs in the miR-1 backbone.

Databases

Tools

Institutional License

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